The intracellular concentration of glutamine is very high where it plays a central role as a ready source ofnitrogen, carbon and energy in various metabolic processes. The purpose of the present study was to investigate theinfluence of intracellular glutamine status on amino acid uptake activity in placental choriocarcinoma (BeWo) cells,analogous to normal placental trophoblasts. Intracellular glutamine was depleted by culturing the cells in regularmedium without glutamine and by treating with 2 mM methionine sulfoximine (MSX), an inhibitor of glutaminesynthetase, for 16 hours. The uptake of various amino acids was measured by the use of appropriate substrates andionic conditions. When cultured in the absence of glutamine and MSX treatment, the uptake of serine, threonine andhistidine was not influenced. Under similar conditions, the uptake of glutamate, alanine, glycine, α-(methylamino)isobutyric acid (MeAIB), taurine and carnitine was reduced to a varied extent. These data showed thatintracellular glutamine was obligatory for maintenance of optimal activity of amino acid uptake in BeWo cells.
"The Depletion of Intracellular Glutamine by Methionine Sulfoximine on Amino Acid Uptake in Placental Cells (BeWo),"
The Thai Journal of Veterinary Medicine: Vol. 42:
2, Article 11.
Available at: https://digital.car.chula.ac.th/tjvm/vol42/iss2/11