The Thai Journal of Veterinary Medicine


The methanolic crude extracts of three weeds, Soapbush or Koster’s Curse (Clidemia hirta), Spanish Flag orWest Indian Lantana (Lantana camara), and Indian rhododendron (Melastoma malabathricum) were studied for theirantibacterial properties. The crude extracts were tested against ten bacterial isolates from giant freshwater prawnlarvae, Macrobrachium rosenbergii. Clidemia hirta extract showed the best MIC and MBC values against Vibrioalginolyticus which were 0.39 mg/ml and 6.25 mg/ml, respectively. Following that, three lowest concentration ofmethanolic crude extracts; 0.195 mg/ml, 0.39 mg/ml, and 0.78 mg/ml, were added to V. alginolyticus culture andobserved for morphological changes via Scanning Electron Microscope (SEM). The cell wall of V. alginolyticus wasdisrupted at 0.195 mg/ml of methanolic crude extract. Antioxidant activities of C.hirta was also studied qualitativelyand quantitatively and compared to the standard reference, Vitamin C. Thin Layer Chromatography (TLC)performed on the C. hirta extract indicated the presence of eight separation compound bands. The phytochemical ofC. hirta extract was positive for tannin, flavonoid, saponin, terpenoid and negative for steroids compounds.

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