The Thai Journal of Veterinary Medicine


The aims of this study were to optimize semen collection by electroejaculation and characterize ejaculatecharacteristics in Malayan tapir (Tapirus indicus). Eight adult males (4-20 years of age) were anesthetized using acombination of medetomidine HCl (0.012-0.018 mg/kg), butorphanol tartrate (0.012-0.018 mg/kg),and ketamine HCl(5-7 mg/kg) administered intramuscularly via a dart and subjected to electroejaculation. Ejaculates (milky-white toyellowish color) were successfully collected from all males. Averages (Mean±SD) of the seminal traits (volume, pH,osmolality, sperm concentration, total sperm motility, and viability) were 10.3±9.4 ml, 7.4±3.4, 278.6±13.3 mOsm,206.2±185.4x106 spermatozoa/ml , 24±15.4%, and 35.1±6.5%, respectively. Morphological analysis showed lowpercentage of normal spermatozoa 6.7±2.6 % with 39.2±8.5% intact acrosomes. This is the first study to evaluatesemen characteristics and sperm morphology in the Malayan tapirs. Results provide fundamental informationessential for development of assisted reproductive technologies in this species.

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