The aim of this study was to determine whether lipid peroxidation is correlated with semen quality in Asianelephant bulls. Malondialdehyde (MDA) in seminal plasma from ejaculates with varying percentages of progressivemotility was measured using Thiobarbituric Acid method. Correlation between the MDA levels and percentages ofprogressive motility and normal morphology were performed. Results revealed that the MDA levels weresignificantly negative, which correlated (p<0.05) with the percentages of progressive motility and normal morphology(R=0.2131 and 0.1685, respectively). The results also showed that the MDA levels were significantly differencebetween each bull (p<0.01). Furthermore, when the ejaculates were grouped according to motility scores into twogroups; low- (<40%) and high-percentage of progressive motility (>40%), a significant difference was detectedbetween the MDA means (±SD) of the low- (20.7±11.4 nmol/ml) and high-percentages of progressive motility(14.4±7.8 nmol/ml) groups. The results obtained from this study suggested that MDA could be a potential parameterapplicable for the assessment of elephant semen quality. It could as well be deduced from this study that oxidativestress might play a key role in low fertility due to poor semen quality in captive male elephants. This data providesbeneficial information to better understanding of elephant reproduction in captivity.
Thongtipsiridech, Sitthawee; Imrat, Podjana; Srihawong, Thanida; Mahasawangkul, Sittidet; Tirawattanawanich, Chanin; and Saikhun, Kulnasan
"Seminal Plasma MDA Concentrations Correlating Negatively with Semen Quality in Asian elephants,"
The Thai Journal of Veterinary Medicine: Vol. 41:
2, Article 10.
Available at: https://digital.car.chula.ac.th/tjvm/vol41/iss2/10