The Thai Journal of Veterinary Medicine
Abstract
A hundred-eighty Escherichia coli isolates from swine were tested for minimal inhibitory concentrations (MICs) of 9 antimicrobials and two heavy metals copper sulfate and zinc chloride. All the E. coli isolates were resistant to at least one antibiotic and 175 (97.2%) isolates were multi-drug resistant. Forty antibiotic resistance patterns were identified, of which the most common pattern was AMP-CHP-CIP-ERY-GEN-STR-SUL-TET-TRI (13.3%). All the isolates formed one large population of susceptibility to copper sulfate and zinc chloride at the concentrations of 6.4-12.8 mM and 3.2-6.4 mM, respectively. Exposure to copper sulfate and zinc chloride at sublethal concentration did not change the susceptibility. Addition of carbonyl cyanide m-chlorophenylhydrazone (CCCP) and reserpine caused at least 4-fold reduction of MIC value of copper sulfate and zinc chloride in 66 (36.7%) isolates and 142 (78.9%) isolates, respectively. The results highlighted the contribution of active efflux systems driven by proton motive force and/or ATP in reduced susceptibilities to copper sulfate and zinc chloride in E. coli from swine.
Publisher
Faculty of Veterinary Science, Chulalongkorn University
First Page
317
Last Page
321
Recommended Citation
Koowatananukul, Chailai; Chansong, Nisit; and Chuanchuen, Rungtip
(2010)
"The Contribution of Active Efflux in Reduced Susceptibilities to Copper Sulfate and Zinc Chloride in Escherichia coli Isolates from Swine,"
The Thai Journal of Veterinary Medicine: Vol. 40:
Iss.
3, Article 11.
Available at:
https://digital.car.chula.ac.th/tjvm/vol40/iss3/11