The Thai Journal of Veterinary Medicine


The aim of the study was to investigate the motility and viability of canine epididymal spermatozoa, collected from the epididymides following orchidectomy (castration), over a period of time. Sperm samples were collected at 25-28oC from the caudal epididymis, 0, 1, 3, 6, and 12 hours, after the castration of 75 fertile dogs (1-10 years old). Progressive motility and viability of the epididymal sperm decreased over a period of time, after the collection. The epididymal sperm motility was 82.3±6, 76.3±8, 72.0±7, 60.3±9, and 22.7±20% while the percentage of viable spermatozoa was 85.5±5, 78.8±7, 77.5±7, 64.4±7, and 27.1±21 at 0, 1, 3, 6 and 12 h, respectively. At 12 h, some samples showed neither motility nor viability, after flushing from the epididymides, while some samples still demonstrated high motility and viability (60-70%). An additional study showed that the motility of epididymal sperm, stored and collected at temperatures higher than 37oC, dramatically decreased within 3 hours. It can be suggested that any delay in epididymal sperm collection results in a deterioration in sperm quality. However, when the epididymis is processed at 25-28oC, within 3 hours, the sperm characteristics remain little affected and the spermatozoa can be used as gamete resources.

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