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The Thai Journal of Veterinary Medicine

Abstract

One hundred clinically healthy Taiwanease soft-shelled turtles (Pelodiscus sinensis) were sampled by blood sampling and cloacal swabing. Samples were first cultured in a pre-enrichment media and then selective isolation was performed for vibrios on Thiosulfate-Citrate-Bile salts-Sucrose agar (TCBS). Species of the isolated vibrios were identified by standard biochemical procedures. A total of 146 vibrio isolates were found in 83 out of 100 cloacal swabs (83%), whereas none were found in the 50 blood samples. Isolates were identified as Vibrio fluvialis (26.04%), V. anguillarum (21.23%), V. furnissii (18.49%), V. parahaemolyticus (18.49%), Vibrio spp. (6.85%), V. damsela (5.48%) and V. metschnikovii (3.42%). This study presented evidence of the presence opportunistic vibrio species in the cloaca of clinically healthy soft-shelled turtles. Vibrio isolates found in these healthy animals correlated with those isolated from feed samples and water from the culture ponds.

Publisher

Faculty of Veterinary Science, Chulalongkorn University

First Page

29

Last Page

35

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