The Thai Journal of Veterinary Medicine


Traditional and molecular genetic typing techniques have been used for discriminating the clonal relationships of bacterial strains. Traditional typing techniques based on phenotypic characteristics such as serotyping are being increasingly replaced by the use of DNA-based methods. The polymerase chain reaction (PCR) has led to typing techniques based on DNA amplification. Randomly amplified polymorphic DNA (RAPD) typing (Arbitrarily Primed-Polymerase Chain Reaction, APPCR) is one of the techniques which is being used increasingly to type micro-organisms, especially during clinical outbreaks. The theoretical aspects and the critical parameters that affect typing and results of RAPD typing are discussed in the text.

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