The Thai Journal of Veterinary Medicine


Coronavirus-like particles were observed by transmission electron microscopy in smooth-walled vesicles of Golgi, or endoplasmic reticulum (ER) in a primary Kupffer cell culture. The culture came from a clinically normal 10-day-old pig, from a swine herd that was known to be infected with porcine respiratory coronavirus (PRCV) and porcine reproductive and respiratory syndrome virus (PRRSV). Budding profiles of virions were observed in the smooth-walled vesicles and later, apparently free viruses accumulated in the collecting vesicles. The virions were enveloped and pleomorphic, with a diameter of 50-100 nm. The virus particles contained an external envelope and an inner membrane and a central core which was 45-85 nm across. Infected-cells underwent swelling with disorganization of the cytoplasmic organelles. Development of secondary lysosomes were common and occasionally contained cell debris with numerous virions and myelin bodies. The coronavirus-like particles in this report may represent PRCV, transmissible gastroenteritis virus (TGEV), or a previously unidentified hepatic coronavirus of pigs.

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