The Thai Journal of Veterinary Medicine


Three monoclonal antibodies (MAbs) against bovine C-reactive protein (CRP) were produced by fusing murine myeloma cell line P3X63Ag8U1 and splenic lympocytes from two BALB/c mice immunized with 100 ug of purified CRP, followed by two booster injections at 10-day intervals. The hybridomas producing antibodies specific for bovine CRP were obtained with effciencies of 1.3 % and 0.2 % respectively. All three MAbs were IgM class with k- light chains. The MAbs obtained from ascitic fluid showed definite antibody activity at 1,000 to 10,000 and the antibody concentrations were 0.9 to 1.7 mg/ml. Two of three MAbs showed significant cross-reactivity with human, goat and cat CRPs, while one reacted with only the homologous CRP antigen. Of 5 MAbs against human CRP (all IgG), only one clone cross-reacted with bovine, goat and cat CRPs, another clone cross-reacted with cat CRP and the remaining three clones showed no cross-reactivity with CRPS of other species.

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