For the assessment of the curative effect of Manosa alliacea on Freud’s adjuvant (FA) arthritis on Swiss albino rats. Methanol extract from M. alliacea (MEMA) was administered orally at 200 mg/kg and 400 mg/kg, 28 days after FA immunization. For control and treatment groups, paw volume, body weight, hematological parameters, X-ray, and histological tests were measured. In addition, reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the levels of various inflammatory markers. In vitro, DPPH and H2O2 tests were used to evaluate the antioxidant capability. MEMA decreased paw volume and paw thickness, bodyweight considerably (P < 0.05, 0.01, 0.001), compared to hematological anomalies of arthritis control. X- rays tests and histological tests did not reveal significant structural changes in the rat ankle joints administered with MEMA. The levels of expression tumor necrosis factor - α, NF-Kβ, IL-1β, and COX-2 were significantly suppressed in the treatment groups. The in-silico study has shown that a number of chemical components in the plants under study can effectively bind to various inflammatory targets. That is why we say M. alliacea is a good source for treating rheumatoid arthritis.
Faculty of Pharmaceutical Sciences, Chulalongkorn University
Prasanth, D. S.N.B.K.; Aminabee, Sk.; Rao, A. Lakshmana; Guntupalli, Chakravarthi; Reddy, Alavala Rajasekhar; Kulandaivelu, Umasankar; Koteswara, S. N.; and Rajeshwari, P.
"Inhibitory effects of Manosa alliacea
in Freund’s adjuvant arthritis on inflammatory markers and its
confirmation by In-silico strategy,"
The Thai Journal of Pharmaceutical Sciences: Vol. 45:
6, Article 12.
Available at: https://digital.car.chula.ac.th/tjps/vol45/iss6/12