The Thai Journal of Pharmaceutical Sciences


Ummaruekvati is an antitussive traditional medicine. It contains licorice as the main ingredient and the major chemical component is glycyrrhizin. The conventional method for quantitative analysis of glycyrrhizin content in licorice is by high-performance liquid chromatography, which is destructive of the sample, time consuming, high cost, and has many steps in the sample preparation process. In this study, a rapid and non-destructive near-infrared spectroscopy (NIR) method was applied for quantitative analysis of glycyrrhizin content in Ummaruekvati dispensatories. A partial least squares chemometric method with various data pre-treatments was performed to establish the calibration model. The best calibration model was obtained using baseline correction over the wavelengths of 1000–2500 nm with four principle components. It showed a high coefficient of determination (R2 = 0.9392), low root mean squared error of cross-validation (RMSECV = 0.0532), and low standard error of calibration (SEC = 0.0516). The average predicted results of glycyrrhizin content in five tested Ummaruekvati dispensatories were 91.74% with the percentage of relative standard deviation of 11.49. The results showed that non-destructive NIR spectroscopic analysis combined with chemometrics can be used for routine quality control of glycyrrhizin content in Ummaruekvati dispensatories with advantages of reduced time with no sample preparation or sample loss.


Faculty of Pharmaceutical Sciences, Chulalongkorn University

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