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The Thai Journal of Pharmaceutical Sciences

Abstract

The present study investigated the phytochemical, high-performance thin-layer chromatography (HPTLC) profiling, total phenolic content, antibacterial activity, acute toxicity studies, hemodynamic parameters, myeloperoxidase (MPO) activity and serum procalcitonin (PCT) levels of hydroalcoholic extract of Bryum weigelii (HE-Bw), and its effects on cecal ligation and puncture (CLP) induced sepsis in mice. The preliminary phytochemical screening showed that HE-Bw contains alkaloids, terpenoids, flavonoids, phenolics, and tannins. The HPTLC analysis of HE-Bw identified three unknown components with different Rf values and area percentage. The total phenolic amount for HE-Bw was equivalent to 211.6 ± 0.8 mg of gallic acid per 100 g of dried plant material. At 100 μg/ml concentration, HE-Bw showed antibacterial activities as potent as streptomycin against Staphylococcus aureus and Escherichia coli. Acute toxicity studies on mice found out that HE-Bw was non-toxic up to 2000 mg/Kg body weight. At both low and high doses, HE-Bw improved hemodynamic parameters such as mean arterial pressure and decreased optical density of blood, while decreased serum MPO activity and PCT levels. Moreover, at a high dose, HE-Bw showed a survival rate of 92.50 ± 3.50% in mice that might be through proinflammatory and bacteremia effects. The results indicate that B. weigelii can be a favorable natural source for CLP-induced sepsis treatment in mice.

Publisher

Faculty of Pharmaceutical Sciences, Chulalongkorn University

First Page

387

Last Page

393

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