The Thai Journal of Pharmaceutical Sciences
Article Title
Moss Bryum weigelii spreng improves survival in septic rats induced by cecal ligation and puncture
Abstract
The present study investigated the phytochemical, high-performance thin-layer chromatography (HPTLC) profiling, total phenolic content, antibacterial activity, acute toxicity studies, hemodynamic parameters, myeloperoxidase (MPO) activity and serum procalcitonin (PCT) levels of hydroalcoholic extract of Bryum weigelii (HE-Bw), and its effects on cecal ligation and puncture (CLP) induced sepsis in mice. The preliminary phytochemical screening showed that HE-Bw contains alkaloids, terpenoids, flavonoids, phenolics, and tannins. The HPTLC analysis of HE-Bw identified three unknown components with different Rf values and area percentage. The total phenolic amount for HE-Bw was equivalent to 211.6 ± 0.8 mg of gallic acid per 100 g of dried plant material. At 100 μg/ml concentration, HE-Bw showed antibacterial activities as potent as streptomycin against Staphylococcus aureus and Escherichia coli. Acute toxicity studies on mice found out that HE-Bw was non-toxic up to 2000 mg/Kg body weight. At both low and high doses, HE-Bw improved hemodynamic parameters such as mean arterial pressure and decreased optical density of blood, while decreased serum MPO activity and PCT levels. Moreover, at a high dose, HE-Bw showed a survival rate of 92.50 ± 3.50% in mice that might be through proinflammatory and bacteremia effects. The results indicate that B. weigelii can be a favorable natural source for CLP-induced sepsis treatment in mice.
Publisher
Faculty of Pharmaceutical Sciences, Chulalongkorn University
First Page
387
Last Page
393
Recommended Citation
Hieu, Ho Viet; Lagu, Surendra Babu; and Tatipamula, Vinay Bharadwaj
(2021)
"Moss Bryum weigelii spreng improves
survival in septic rats induced by cecal
ligation and puncture,"
The Thai Journal of Pharmaceutical Sciences: Vol. 45:
Iss.
5, Article 10.
Available at:
https://digital.car.chula.ac.th/tjps/vol45/iss5/10