Chulalongkorn University Dental Journal

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Objective To evaluate the effect of fluoride/zinc/essential oil-containing mouthrinse on fluoride uptake and microhardness in enamel. Materials and methods A double-blind randomized trial was performed. Thirty polished bovine enamel specimens (6 x 6 mm) were subjected to caries-likes lesion formation in the demineralizing solution. Then specimens were analyzed for microhardness (no difference in microhardness; p > 0.05) and divided into 3 groups (n=10/group). The fluoride and calcium contents in enamel were analyzed by fluoride electrode and atomic absorption spectrometer, respectively. The specimens in three groups were then treated with either fluoride/zinc/essential oil-containing mouthrinse (Listerine Total Care-LTC), 100 ppm NaF (positive control), or 5% ethanol (negative control) and subjected to 30 cycles of demineralization/remineralization ie. 10 min in demineralizing solution, 5 min in test solution, and 60 min in remineralizing solution. After the cycling, the fluoride, calcium and microhardness in enamel were determined. The changes of fluoride content (Δ F) and microhardness (Δ H) after treatment were analyzed between groups using a one way ANOVA and Tukey test. Results The Δ F obtained from the test mouthrinse (71.10 ± 45.50 ppm) was not statistically different (p > 0.05) from that of 100 ppm NaF (41.41 ± 26.75 ppm). However, both solutions showed significantly more fluoride uptake than 5% ethanol group (0.20 ± 2.30 ppm) (p < 0.05). The Δ H of LTC treated enamel (6.7 ± 2.8 Vickers) was not different (p > 0.05) from that of 100 ppm NaF treated samples (10.9 ± 6.1 Vickers). However, Δ Hs of both treatments were significantly more than in ethanol treated teeth (1.1 ± 1.7 Vickers) (p < 0.05). Conclusion Fluoride/zinc/essential oil-containing mouthrinse can promote enamel fluoride uptake and microhardness.



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