Chulalongkorn University Dental Journal

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Objective To investigate the effects of dental radiation on the expression of apoptotic-related genes in primary human bone cells. Materials and methods Human bone cells were grown in 15% FBS-DMEM and irradiated with 0, 1, 2 doses of a periapical radiograph. The cytotoxicity of irradiation was investigated by MTT assay after 24 hours. The levels of apoptotic-related gene expressions, Bcl-2, Bax, Bad, Bcl-xL, caspase-3 were analyzed by reverse transcription polymerase chain reaction, four hours after irradiation. Results No cytotoxicity was observed at the time of 24 hours after dental irradiation. The results indicated that Bax and caspase-3 significantly decreased after irradiation as compared to the control group, whereas, the expression of Bcl-2, Bad and Bcl-xL did not change significantly after dental irradiation. Conclusion Dental radiation affects apoptotic-related gene expressions. Therefore, prescribing dental radiograph should be highly selective and the principle of ALARA (As Low As Reasonably Achievable) should be strictly followed.



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