Chulalongkorn University Dental Journal

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Objective Sporicidal activity is a good indicator for testing the efficacy of chemical disinfectants in infection control. We tested the sporicidal effect of common germicides used in Thailand. Materials and methods The spore-forming bacteria Bacillus atropheas and Geobacillus stearothermophilus were chosen to test four commercial chemical agents: sodium hypochlorite, 2% glutaraldehyde, 35% hydrogen peroxide, and iodophore. Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella typhi were also used to ensure compliance with EPA (Environmental Protection Agency) and AOAC (Association of Analytical Communities) laboratory standards for hospital disinfectants. Results Two percents glutaraldehyde inactivated B. atropheas and G. stearothermophilus at 60 and 30 min, respectively; while 0.25% sodium hypochlorite (wt/v) killed the spores at 20 and 30 min. Thirty five percents hydrogen peroxide was effective at 5 min, iodophore at 0.007% concentration (w/v) inactivated G. stearothermophilus but not B. atropheas spores. Conclusion The commercially available chemical agents: 2% glutaraldehyde, 0.25% sodium hypochlorite and 35% hydrogen peroxide for dental practice in Thailand have sporicidal effects when used as manufacturer suggested; thus, they can be used as high level disinfectants.



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