Chulalongkorn University Dental Journal
Publication Date
2007-09-01
Abstract
Objective To clone human connective tissue growth factor (hCTGF) from pulpal fibroblasts. Materials and methods Primary human pulpal fibroblasts were isolated from pulpal tissues. The reverse transcriptase-PCR assay was used to amplify the cDNA of hCTGF. The amplified PCR products were ligated into the TOPO R cloning vector and transformed into competent bacteria cells. The putative clones were bidirectionally sequenced to analyze nucleotide sequence and compare with hCTGF cDNA sequence references. Results From RT-PCR reaction, expression of CTGF mRNA was detected in human pulpal fibroblast. Through bi-directional sequencing analysis, nucleotide sequence of our hCTGF has 100% homology to the hCTGF sequence reported. Conclusion Human pulpal fibroblasts express CTGF mRNA. The hCTGF cDNA obtained from primary pulpal fibroblast has 100% homology to hCTGF sequence references.
DOI
10.58837/CHULA.CUDJ.30.3.1
First Page
227
Last Page
234
Recommended Citation
Thunyakitpisal, Pasutha
(2007)
"cDNA cloning and nucleotide sequencing of human connective tissue growth factor obtained from primary pulpal fibroblasts,"
Chulalongkorn University Dental Journal: Vol. 30:
Iss.
3, Article 1.
DOI: 10.58837/CHULA.CUDJ.30.3.1
Available at:
https://digital.car.chula.ac.th/cudj/vol30/iss3/1