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Chulalongkorn University Dental Journal

Publication Date

2007-01-01

Abstract

Objective The purpose of this study was to examine the antibacterial activity of extract from mangosteen pericarp against Streptococcus mutans, bacteria associated with dental plaque formation and caries development. Materials and methods Bacterial strains used in this study were S. mutans ATCC 25175 and KPSK2. Antibacterial activity was expressed as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The rates at which bacteria were killed were also determined by exposure to the extract at 2x and 4x MBC for varying time periods. Results The extract was effective against both strains of S. mutans. The MIC and MBC for the strain ATCC 25175 were both equal to 0.625 μg/ml. The respective values for the strain KPSK2 were 0.625 and 1.25 μg/ml. The MIC and MBC were comparable to those of chlorhexidine, an antiseptic commonly used in dental plaque control. Time-kill assays for the strain ATCC 25175 showed that treatment with the extract at 2x MBC caused a significant decrease in viable count (log10 colony forming units/ml) of almost one order of magnitude after 90 minutes. When the extract concentration was increased to 4x MBC, the viable bacterial count was reduced by almost two orders of magnitude in 60 minutes, and was further decreased to an undetectable level in 90 minutes. Conclusion Mangosteen pericarp extract possessed strong antibacterial activity against S. mutans. These results suggest that the extract may be developed as a new agent for dental plaque control and caries prevention.

DOI

10.58837/CHULA.CUDJ.30.1.1

First Page

1

Last Page

10

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