Chulalongkorn Medical Journal


Background: Antioxidants from natural sources are well-accepted for reducing oxidative damage and preventingthe development of oxidative stress-related diseases. It is known that urinary stone development involvesoverproduction of reactive oxygen species and accumulation of oxidative injury. Supplements with antioxidantshad been shown to inhibit kidney stone formation in animal models.Objective: We developed a new beverage, called HydroZitLa (concentrate in pouch) to be used for preventionof urinary stone formation. HydroZitLa consisted of citrate (16 mEq) and naturally occurring antioxidants fromThai medicinal plants.Methods: We investigated the total phenolic content (TPC), total flavonoid content (TFC), and total antioxidantcapacity (TAC) in HydroZitLa. Also, cytotoxicity of HydoZitLa in HK-2 cells was evaluated.Results: TPC and TFC in HydroZitLa were 10.2  0.5 mg galic acid equivalents (GAE) pouch and 2.8  0.2 mgcatechin equivalent (CE) pouch, respectively. TAC in HydroZitLa determined by 2,2'-azino-bis (3-ethylbenzthiazoline6-sulphonic acid)(ABTS) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assays were 12.6  0.2 and11.3  0.3 mg vitamin C equivalent antioxidation capacity (VCEAC) pouch, respectively. Stability test data at day4 revealed that TPC and TAC (by DPPH) in HydroZitLa kept at 4C were significantly higher than room temperature.Cytotoxicity test showed that HydroZitLa at 10% (v/v) and higher concentrations significantly caused reductionof HK-2 cell viability, and IC50 of HydroZitLa was of 24.6% (v/v).Conclusion: TPC, TFC and TAC in HydroZitLa were considerably high. Natural antioxidants together withcitrate in HydroZitLa had a potential to prevent the formation of urinary calculi. Further pre-clinical and clinicalstudies should be conducted to warrant the therapeutic action of HydroZitLa in urolithiasis.


Faculty of Medicine, Chulalongkorn University

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