Background : Genetic polymorphisms of protein transporters can alter the pharmacokineticsand pharmacodynamics of several substrate drugs including antiretrovirals(ARVs). The polymorphisms of ABCB1 and SLCO1B1 have been investigatedin Caucasian and some of Asian populations. However, the information ofthese single nucleotide polymorphisms (SNPs) in Thai population are limited.Objective : The aim of this study was to determine the frequencies of the variantgenotype and allele of ABCB1 and SLCO1B1 in Thai HIV-infected patientsand to compare with the frequencies obtained from other populations.Methods : Three SNPs: ABCB1 3435C>T, ABCB1 2677G>T and SLCO1B1 521T>Cwere identified by Taqman allelic discrimination assays using real timepolymerase chain reaction system. Chi-square test was performed tocompare the distributions of observed genotypes with expected genotypesaccording to Hardy-Weinberg equilibrium and to compare the proportionsof allele frequencies between our population and other ethnic populations.Results : A total of 521 patients were included in this study. The homozygousvariant genotypes were 22.50%, 29.75%, and 3.84% for ABCB1 3435TT,ABCB1 2677TT and SLCO1B1 521CC, respectively. The allele frequenciesof ABCB1 3435C>T, ABCB1 2677G>T and SLCO1B1 521T>C were 47.70%,51.06%, and 11.90%, respectively. The prevalence of ABCB1 2677G>Twas similar to other Asian populations, but the prevalence of ABCB13435C>T was different from the Chinese and the Korean populations.In addition, higher variant allele frequencies of ABCB1 3435T, ABCB12677T and SLCO1B1 521C were observed in Thais compared to AfricanAmericans.Conclusion : We found that gene polymorphisms of ABCB1 and SLCO1B1 were frequentin Thai HIV-infected patients. These results could provide an evidence forfuture clinical studies regarding the variability in treatment responses anda risk of adverse effects of ARVs which are substrate of these transportersin Thai population.
Faculty of Medicine, Chulalongkorn University
Singkham, Noppaket; Avihingsanon, Anchalee; Bunupuradah, Torsak; and Punyawudho, Baralee
"Genotype and allele frequencies of ABCB1 and SLCO1B1 polymorphisms in THAI HIV-infected patients,"
Chulalongkorn Medical Journal: Vol. 62:
4, Article 7.
Available at: https://digital.car.chula.ac.th/clmjournal/vol62/iss4/7