Objective : To study Blastocystis hominis infection, positivity by using Boeck andDrbohlav Locke egg serum medium (LE) cultivation and Jones’ medium.Design : Cross-sectional study.Setting : Bann Kew Savag Mai, Mae Chame District, Chiang Mai Province andDepartment of Parasitology, Faculty of Medicine, Chulalongkorn University,Bangkok, Thailand.Materials : One hundred and fifty-eight stool specimens from hill-tribe children, aged1-18 years old.Methods : All 158 stool specimens were cultivated in Jones’ and LE media, transportedto the laboratory and examined for B.hominis under the microscope.The parasites were detected after a 72 hr incubation at 37C. Each stoolsample was also identified for B.hominis by simple smear and concentrationtechniques.Results : The prevalence rate of B.hominis was 0.91% and 1.09%, examined bysimple smear and formalin ether concentration technique, respectively.The Jones’ medium demonstrated 22.15% parasite positivity. The LEmedium showed a high efficiency of organism detection with 43.67%parasite positivity.Conclusion : The classical LE medium which is routinely used to detect the presence ofintestinal amoeba, primarily Entamoeba histolytica in many hospital-basedlaboratories has no available report for B.hominis detection. In this study,the standard LE medium performed the most efficiently for B.hominisproduction when compared to Jones’ medium. The LE medium may beused as a reliable medium which has low-cost. This medium can eliminatethe problem of the expiration date of the yeast extract used in the Jones’medium.
Faculty of Medicine, Chulalongkorn University
Saksirisampant, W; Nuchprayoon, S; Pradniwat, P; and Lamchuan, D.
"Boeck and Drbohlav Locke egg serum medium fordetection of Blastocystis hominis,"
Chulalongkorn Medical Journal: Vol. 54:
6, Article 1.
Available at: https://digital.car.chula.ac.th/clmjournal/vol54/iss6/1