Chulalongkorn University Theses and Dissertations (Chula ETD)

Microemulsion electrokinetic chromatography for separation and analysis of avermectin macrocyclic lactones

Other Title (Parallel Title in Other Language of ETD)

ไมโครอิมัลชันโทรไคเนทิกโครมาโทกราฟีสำหรับการแยกและการวิเคราะห์อะเวอร์เมกทินแมกโครไซคลิกแลกโทน

Year (A.D.)

2005

Document Type

Thesis

First Advisor

Thumnoon Nhujak

Faculty/College

Graduate School (บัณฑิตวิทยาลัย)

Degree Name

Master of Science

Degree Level

Master's Degree

Degree Discipline

Chemistry

DOI

10.58837/CHULA.THE.2005.2102

Abstract

Microemulsion electrokinetic chromatography (MEEKC) was developed for quantitative analysis of avermectins, such as abamectin, doramectin and ivermectin, in commercial formulations, using the microemulsion buffer containing a 50 mm phosphate buffer at pH 2.5, 1.1 % v/v n-octane as oil droplets, 180 mM sodium dodecyl sulfate as surfactant, 890 mM 1-butanol as co-surfactant and 30 % v/v ethanol as organic co-solvent; applied voltage of -15 kV; and separation temperature at 25 °C. Achieved baseline resolution up to 4.9 was obtained with analysis time within 25 min. High accuracy and precision of the method were obtained. The developed MEEKC method was used for quantitative analysis of avermectins in commercial formulations. The contents of avermectins in commercial formulations determined by MEEKC were found to be insignificantly different with those determined by high performance liquid chromatography (HPLC). Therefore, MEEKC can be used as an alternative method to HPLC for quantitative determination of avermectins with shorter analysis time and lower amount of organic solvent

Other Abstract (Other language abstract of ETD)

yMicroemulsion electrokinetic chromatography (MEEKC) was developed for quantitative analysis of avermectins, such as abamectin, doramectin and ivermectin, in commercial formulations, using the microemulsion buffer containing a 50 mm phosphate buffer at pH 2.5, 1.1 % v/v n-octane as oil droplets, 180 mM sodium dodecyl sulfate as surfactant, 890 mM 1-butanol as co-surfactant and 30 % v/v ethanol as organic co-solvent; applied voltage of -15 kV; and separation temperature at 25 °C. Achieved baseline resolution up to 4.9 was obtained with analysis time within 25 min. High accuracy and precision of the method were obtained. The developed MEEKC method was used for quantitative analysis of avermectins in commercial formulations. The contents of avermectins in commercial formulations determined by MEEKC were found to be insignificantly different with those determined by high performance liquid chromatography (HPLC). Therefore, MEEKC can be used as an alternative method to HPLC for quantitative determination of avermectins with shorter analysis time and lower amount of organic solvent

ISBN

9745326631

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