Chulalongkorn University Dental Journal
Publication Date
2015-09-01
Abstract
Objective The aim of this study was to compare the efficiency of Mitis salivarius bacitracin agar that were stored for 1,3 and 4 weeks Materials and methods The paraffin stimulated saliva samples were collected from 20 individuals. The samples were plated onto MSA and MSB media that were stored at 4°C for 1, 3 and 4 weeks by spread plate method. Results The viable count of mutans streptococci on Mitis salivarius bacitracin agar medium that were stored at 4°C for 1, 3 and 4 weeks were not significantly different. Mutans streptococci grown on these media displayed typical colony morphology and there was no outgrowth of other species. The viable count of bacteria was significantly higher in Mitis salivarius agar media without bacitracin than those with bacitracin media (p < 0.05). Conclusion Mitis salivarius bacitracin agar medium containing high sucrose (20%) can be stored at 4°C for as long as 4 weeks without losing selectivity for mutans streptococci groups.
DOI
10.58837/CHULA.CUDJ.38.3.1
First Page
177
Last Page
184
Recommended Citation
Yapong, Bunjird; Tunnukit, Sissada; and Jitpukdeebodintra, Suwanna
(2015)
"Efficiency of Mitis salivarius bacitracin agar stored for 4 weeks for isolation of mutans streptococci from human saliva,"
Chulalongkorn University Dental Journal: Vol. 38:
Iss.
3, Article 1.
DOI: 10.58837/CHULA.CUDJ.38.3.1
Available at:
https://digital.car.chula.ac.th/cudj/vol38/iss3/1