Effect of acemannan on the dentinsialophosphoprotein and dentin matrix protein 1 mRNA expressions in primary human pulpal cells

Authors

Pechanika Lardungdee
Pornpun Asvanit
Pasutha Thunyakitpisal

Publication Date

2008-09-01

Abstract

Objective To investigate the effect of acemannan isolated from Aloe vera gel on the steady level of dentinsialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP 1) mRNAs in primary human pulpal cells. Materials and methods Cells were treated with the designated concentrations of acemannan (0.25, 0.5 and 1 mg/ml) for 24 hours. The reverse transcription-polymerase chain reaction assays were used to investigate the effects of acemannan on the steady level of DSPP and DMP 1 mRNAs. Results At 24 hours of incubation, acemannan (0.5 mg/ml) significantly enhanced the expressions of DSPP and DMP 1 mRNA levels up to 1.93 and 2.76 fold, respectively, as compared with the control group (p < 0.05). Conclusion Acemannan at concentration 0.5 mg/ml stimulated both DSPP and DMP 1 mRNA expressions in human dental pulp cells.

DOI

10.58837/CHULA.CUDJ.31.3.13

First Page

407

Last Page

414

Recommended Citation

Lardungdee, Pechanika; Asvanit, Pornpun; and Thunyakitpisal, Pasutha (2008) "Effect of acemannan on the dentinsialophosphoprotein and dentin matrix protein 1 mRNA expressions in primary human pulpal cells," Chulalongkorn University Dental Journal: Vol. 31: Iss. 3, Article 13.
DOI: 10.58837/CHULA.CUDJ.31.3.13
Available at: https://digital.car.chula.ac.th/cudj/vol31/iss3/13