The effect of 500 and 1000 ppm fluoride dentifrices on growth of cariogenic bacteria: in vitro study

Authors

Pimpilai Limsomwong
Watcharaporn Tasachan
Patchara Pipattanagovit

Publication Date

2008-09-01

Abstract

Objective The purpose of this study was to compare antimicrobial effect of 500 and 1000 ppm fluoride dentifrices that distributed in Thailand. Materials and methods Streptococcus mutans (ATCC 25175), Streptococcus sobrinus (OMZ 176a) and Lactobacillus casei (IFO 3533) were involved in this study. Fluoride dentifrices brought from the local market, which six dentifrices were containing 500 ppm fluorides and six dentifrices were containing 1000 ppm fluoride. Supernatants from each were prepared, soluble fluoride ion was analyzed by fluoride electrode, and bring to test against bacteria in agar plates by agar diffusion method. The diameter of the bacterial zone of inhibition was measurement by Image Pro Plus® program (version 4.5) and calculated to bacterial inhibition area. The data were analyzed by SPSS (version 13) to determine the differences among the means at the significance level of p = 0.05. Results The results show that there were no significant differences among the mean bacterial inhibition zone of 500 ppm fluoride dentifrices (p > 0.05). In contrast, there were statistically significant differences among mean bacterial inhibition zone of 1000 ppm fluoride dentifrices (p < 0.05). When compare between 500 and 1000 ppm fluoride dentifrices, there was a statistically significant difference (p < 0.05). Conclusion 1000 ppm fluoride dentifrices have more antimicrobial effect than 500 ppm fluoride dentifrices. In addition, the quantity of soluble fluoride ions from fluoride dentifrices has correlation with antimicrobial effect.

DOI

10.58837/CHULA.CUDJ.31.3.11

First Page

385

Last Page

398

Recommended Citation

Limsomwong, Pimpilai; Tasachan, Watcharaporn; and Pipattanagovit, Patchara (2008) "The effect of 500 and 1000 ppm fluoride dentifrices on growth of cariogenic bacteria: in vitro study," Chulalongkorn University Dental Journal: Vol. 31: Iss. 3, Article 11.
DOI: 10.58837/CHULA.CUDJ.31.3.11
Available at: https://digital.car.chula.ac.th/cudj/vol31/iss3/11