The antimicrobial effect against Streptococcus mutans of glass-ionomer cement containing polysaccharide gel from durian fruit-hulls

Authors

Suthapar Sri-arunotai
Paiboon Techalertpaisarn
Pasutha Thunyakitpisal
Sunan Pongsamart

Publication Date

2008-05-01

Abstract

Objective To investigate the antimicrobial effect of glass-ionomer cement containing polysaccharide gel from durian fruit-hulls against Streptococcus mutans in vitro. Materials and methods The powder mixtures of 4.76, 7.50, 9.09 and 12.50 percent by weight of polysaccharide gel from durian fruit-hulls and glass-ionomer cement were prepared as experimental groups. Then, the test substances were mixed with the liquid part following the manufacturerûs instructions and poured in the cylindrical brass mold with 3 mm in diameter and 2 mm in height. Each group was composed of 6 specimens. Glass-ionomer cement alone was used as a negative control group, while a mixture of 4.76 percent by weight of ampicillin and glass-ionomer cement was used as a positive control group. The antimicrobial activity was determined by using the broth dilution technique. Each specimen was dropped in Trypticase Soy Broth contained with 1 x 105 CFU/ml of S. mutans. Subsequently, they were incubated at 37 °C, 5% CO2 for 4, 8 and 24 hours. Their survival numbers were evaluated in comparison to the control groups using the drop plate method. Results At 8 hours incubation period, glass-ionomer cement with polysaccharide gel from durian fruit-hulls for 7.50, 9.09 and 12.50 percent established significant inhibitory effects (approximately 15 percent) against S. mutans (P < 0.05). At 24 hours, they had approximately 5 percent inhibitory effects against S. mutans (P > 0.05), whereas, glass-ionomer cement and glass-ionomer cement with 4.76 percent polysaccharide gel from durian fruit-hulls showed no inhibitory effect against S. mutans. Conclusion The glass-ionomer cement with 7.50, 9.09 and 12.50 percent by weight polysaccharide gel from durian fruit-hulls for showed significant inhibitory effects against S. mutans at 8 hours incubation.

DOI

10.58837/CHULA.CUDJ.31.2.7

First Page

193

Last Page

200

Recommended Citation

Sri-arunotai, Suthapar; Techalertpaisarn, Paiboon; Thunyakitpisal, Pasutha; and Pongsamart, Sunan (2008) "The antimicrobial effect against Streptococcus mutans of glass-ionomer cement containing polysaccharide gel from durian fruit-hulls," Chulalongkorn University Dental Journal: Vol. 31: Iss. 2, Article 7.
DOI: 10.58837/CHULA.CUDJ.31.2.7
Available at: https://digital.car.chula.ac.th/cudj/vol31/iss2/7