Chulalongkorn University Dental Journal
Publication Date
2008-01-01
Abstract
Objectives To study the response including cell proliferation and cell attachment of human bone marrow stromal cells to collagen and osteopontin in vitro. Materials and methods The study was performed by co-culturing human bone marrow stromal cells with bovine dermal collagen and recombinant rat osteopontin. MTT assay was utilized to determine the cell proliferation and cytotoxicity resulted from the cells being exposed to four conditioned surfaces, uncoated, collagen-coated, osteopontin-coated and mixed collagen/osteopontin-coated. Cell attachment to these four conditioned surfaces was also investigated under the scanning electron microscope. Results The cells exposed to the collagen-coated and the mixed collagen/osteopontin-coated surfaces demonstrated the increasing of cell proliferation to 106.52 ± 4.08% and 114.78 ± 6.82% respectively, whereas the cells exposed to the osteopontin-coated surface revealed the decrease in cell number to 53.48 ± 12.20% when compared to the control group. Scanning electron microscopy showed good cell attachment in all studied groups. Conclusion Both collagen containing solutions enhanced the human bone marrow stromal cell proliferation and attachment. The enhancement is increased with the addition of osteopontin. These results suggest that collagen and osteopontin are advantageous to the bone marrow stromal cells. Therefore, it might be worth introducing them to the field of bone regeneration.
DOI
10.58837/CHULA.CUDJ.31.1.3
First Page
19
Last Page
32
Recommended Citation
Sirikururat, Papatpong; Tamsailom, Suphot; Chang, Pi-Ling; and Swasdison, Somporn
(2008)
"The response of human bone marrow stromal cells to osteopontin and bovine dermal collagen: an in vitro study,"
Chulalongkorn University Dental Journal: Vol. 31:
Iss.
1, Article 3.
DOI: 10.58837/CHULA.CUDJ.31.1.3
Available at:
https://digital.car.chula.ac.th/cudj/vol31/iss1/3