Antimicrobial activity of polysaccharide gel from durian fruit-hulls against Streptococcus mutans and Actinobacillus actinomycetemcomitans

Authors

Phakawan Musikapong
Pasutha Thunyakitpisal
Sunanta Pongsamart

Publication Date

2005-05-01

Abstract

Objective To investigate the antimicrobial activity of polysaccharide gel (PG) extracted from fruithulls of durian against Streptococcus mutans and Actinobacillus actinomycetemcomitans, in vitro. Materials and methods An inhibitory activity was determined by using broth dilution technique and time kill analysis. S. mutans and A. actinomycetemcomitans were grown in Tryptic Soy Broth and Brain Heart Infusion, respectively. Both organisms were treated with different concentrations of PG (1, 5, 10, 20 and 35 mg/ml) in broth medium at 37˚C, 5% CO2 for 24 hours. Their survival were evaluated in comparison to the control using drop plate method. Results The minimum inhibitory concentrations (MICs) of S. mutans and A. actinomycetemcomitans were 20 and 15 mg/ml, respectively, while the minimum bactericidal concentration (MBC) of S. mutans and A. actinomycetemcomitans was 35 mg/ml. Bacterial counts of both S. mutans and A. actinomycetemcomitans survival were declined to zero at 4 hours incubation in medium with 35 mg/ml PG. Conclusion PG, at 35 mg/ml, revealed the bactericidal activities against both S. mutans and A. actinomycetemcomitans. PG, at 20 and 15 mg/ml, exhibited an inhibitory activities against S. mutans and A. actinomycetemcomitans, respectively.

DOI

10.58837/CHULA.CUDJ.28.2.6

First Page

137

Last Page

144

Recommended Citation

Musikapong, Phakawan; Thunyakitpisal, Pasutha; and Pongsamart, Sunanta (2005) "Antimicrobial activity of polysaccharide gel from durian fruit-hulls against Streptococcus mutans and Actinobacillus actinomycetemcomitans," Chulalongkorn University Dental Journal: Vol. 28: Iss. 2, Article 6.
DOI: 10.58837/CHULA.CUDJ.28.2.6
Available at: https://digital.car.chula.ac.th/cudj/vol28/iss2/6