Effect of simvastatin on the proliferation and enzyme activity of MMP-2 and -9 in cancer cell lines HSC-4 and CCL-25

Authors

Risa Chaisuparat
Phakawan Musikapong
Pasutha Thunyakitpisal

Publication Date

2004-01-01

Abstract

Objective To study the effect of simvastatin on the proliferation and enzyme activity of MMP-2 and -9 in HSC-4 and CCL-25 cancer cell lines Materials and methods Cells were treated with simvastatin in the serum-free media for 24 hours. The number of cells were measured by MTT technique. Enzyme activities in the conditioned media were measured by gelatin zymography. The data were analyzed by One way ANOVA (α<0.05). Results Simvastatin, at 15 and 20 µM, significantly decreased the proliferation of HSC-4 and CCL-25 compared with the control group. Simvastatin, at 10 µM, significantly decreased the MMP-9 activity in CCL-25. Simvastatin did not significantly effect the MMP-2 and MMP-9 activities in HSC-4. Conclusion In HSC-4 and CCL-25, the toxicity concentration of simvastatin is 15 and 20 µM. Simvastatin, at 10 µM, significantly suppressed MMP-9 activity in CCL-25.

DOI

10.58837/CHULA.CUDJ.27.1.6

First Page

59

Last Page

67

Recommended Citation

Chaisuparat, Risa; Musikapong, Phakawan; and Thunyakitpisal, Pasutha (2004) "Effect of simvastatin on the proliferation and enzyme activity of MMP-2 and -9 in cancer cell lines HSC-4 and CCL-25," Chulalongkorn University Dental Journal: Vol. 27: Iss. 1, Article 6.
DOI: 10.58837/CHULA.CUDJ.27.1.6
Available at: https://digital.car.chula.ac.th/cudj/vol27/iss1/6