Inhibitory effect of EGCG of EGCG on collagen induced MMP-2 activation in oral squamous carcinoma cell lines

Authors

Neeracha Sanchavanakit
Amornrat Suwanchai
Orachatr Boonyakiati
Artiphan Pimkhaokham
Prasit Pavasant

Publication Date

2003-01-01

Abstract

Objective The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG), the major polyphenol compound of the tea extract on the inhibition of matrix metalloproteinase (MMP)-2 activation in oral squamous carcinoma cell lines. Materials and methods The study was performed in the cultures of human squamous carcinoma (HSC) -3 and 7 and human foreskin fibroblast (HFF). EGCG at 0, 1 and 10 µM was added to the 200 µg/ml collagen induced MMP-2 activation in these cell lines of 48 hours. Conditioned media were collected and MMP-2 activation was analyzed by gelatin zymography. Result Ten µM EGCG reduced the activation of MMP-2 in HSC-3 and HFF, whereas inhibited that of MMP-2 in HSC-7. Conclusion The result suggested another role of EGCG in the reduction of MMP-2 activation, which was implicated in cancer metastatic process, apart from an antioxidant effect on carcinogenesis that has been reported in various types of cancer.

DOI

10.58837/CHULA.CUDJ.26.1.1

First Page

1

Last Page

14

Recommended Citation

Sanchavanakit, Neeracha; Suwanchai, Amornrat; Boonyakiati, Orachatr; Pimkhaokham, Artiphan; and Pavasant, Prasit (2003) "Inhibitory effect of EGCG of EGCG on collagen induced MMP-2 activation in oral squamous carcinoma cell lines," Chulalongkorn University Dental Journal: Vol. 26: Iss. 1, Article 1.
DOI: 10.58837/CHULA.CUDJ.26.1.1
Available at: https://digital.car.chula.ac.th/cudj/vol26/iss1/1